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番茄组培苗的不同阶段对抗生素和PPT的抗性筛选试验
entmass—concentrationsduringthestagesofthecallusformationoftomato’Scotyledonandhypoeotyl，thedifferentiationandgrowthoftomato’Sbud，andtheroot—growthoftomatopla—nfle~，SOthattheoptimumscreeningmass—concentrationsneededbygenetictransformationoftomatocouldbedetermined．Theexperimentshowedthattherewasnotdistinctdifferenceinresistancetoabovereagentsbetweenthetwoself-bredlints—T79andT151；ateachstageoftomatotissueculture。bethkanamyeinandPPrrhadaverystrongerinhibitiononexplants．whileneomycinhadnoobviousinhibitiononthem,[link widoczny dla zalogowanych]；ontheotherhand,[link widoczny dla zalogowanych]，duringthedifferentstages，thereexistedobviousdifferenceindifferentexplants’reactiontokanamyeinandPPrr，andparticularlythecallusformationofhypoeotylWasthemostsusceptibletoKan。thoughthecallusformationofcotyledonandtheroot—growthofplantletswereunsusceptibletoit；inaddition，thecallusformationofhypoco~andthedifferentiationofbudsweresusceptibletoPIyr,[link widoczny dla zalogowanych]，thoughthecallusformationofcotyledonWasunsusceptibletoit．Hence，itWassuggestedthatforgenetictransfor-mationoftomato，Kan100nag,[link widoczny dla zalogowanych]。L～shouldbeselectedasidealscreeningmass—concentrationatthestageofhypoeotylcallusinducingandplantlets’root—growth．Kan50nag。L～atthestageofhypoeotylcallusin—ducing；andKan70mg。L～atthestageofbuddifferentiation．However。forPPT，1．0mg·L一。shouldbetakenasidealscreeningmass—concentrationatthestageofcotyledoncallusinducing。0．5mg。L一。atthestageofhypoeotylcallusinducing；and0．75mg。Latthestageofbuddifferentiationandplanflets’root—growth．Keywords：tomato；tissueculture；pre．testinresistance,[link widoczny dla zalogowanych]；antibiotics；herbicidePPrr
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