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Bacterial contamination of platelets Analysis


(Continued from 4626) 3 to discuss the sensitivity and specificity of detection reagents continuous improvement and continuous improvement of virus detection methods, risk of transfusion transmitted virus significantly reduced. But with the increasing amount of platelet transfusion reaction caused by bacterial contamination have been reported. According to the blood monitoring system, information obtained in 2000. Transfusion led to the U.S. Top 5 causes of death were: acute hemolytic transfusion reactions,[link widoczny dla zalogowanych], bacterial contamination reactions, transfusion related acute lung injury,[link widoczny dla zalogowanych], non-bacterial infections and transfusion-associated graft-versus-host disease. During the period from 1994 to 1998, the French surveillance system found 31 cases of blood and transfusion-related deaths in patients with bacterial pollution l8 _1]. Shows the severity of bacterial contamination of transfusion reactions. Bacterial contamination of platelets in different countries have different test results, which confirm the positive rate of 0.02% -1.8% [. We have detected that the positive rate was 0.12%, close to the monitoring results of Hong Kong (0.05% ~ 0.1%)]. Caused by bacterial contamination of platelets are mainly the following ways: �� bacteria exist in the deep parts of the skin, easy to disinfect,[link widoczny dla zalogowanych], through the needle into the blood; �� bacteremia in patients with blood donors themselves; �� When blood is not in strict accordance with standard operation; �� blood collection equipment and other inherent microleakage _4]. Therefore, to reduce bacterial contamination of transfusion reactions. For different ways to take corresponding measures, such as strict disinfection before blood collection: the first collection of 10 ~ 15llll used for the detection of blood or discard: Strengthening the screening of blood donors before, rule out the potential bacteremia in patients with blood donation: blood strict inspection before the integrity of packaging and so on. However, platelets were collected and need to save under the conditions of 22 ��. Bacteria reproduced,[link widoczny dla zalogowanych], even though it is difficult to avoid the above platelet transfusion reaction caused by the occurrence of bacterial contamination. Therefore,[link widoczny dla zalogowanych], during storage of platelet bacterial contamination of its monitoring may be an effective method. Present. Detection of bacterial contamination There are many ways. Including the naked eye visual inspection, direct smear, direct culture, antigen antibody, nucleic acid hybridization probe of antibiotics, such as a bacterial endotoxin test]. However, these methods require a certain concentration of bacteria to (> 10cfu) only when detected. Microbial monitoring system with Bact/Alert3D monitoring of bacteria, due to nutrient-rich medium, the bacteria reproduce in the culture bottle in the blood faster than the speed. Microbiological surveillance system by Bact/Alert3D situation flask, to more sensitive detection of the presence of bacteria [33: laboratory conditions by Bact / Alea systems and standards can be detected in platelet concentrates flask very low concentrations of bacteria (< ; 1cfu/m1) vii. But this method also has certain disadvantages, such as reagents, equipment, more expensive, take longer and so on, to the conventional bacterial culture of platelet bring some difficulties
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