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Extraction of total glucosides of Cordyceps


Evaporated to dryness in a water bath, dried at 105 ℃ to constant weight. The total yield of glycosides / a [Cordyceps sinensis extract powder dry mass (g) / Cordyceps powder quality (g)] × 1O0. 2.2 Ultrasonic extraction alcohol orthogonal in Table 1. Take Cordyceps powder 4g, with methanol as extraction solvent, extraction by orthogonal ultrasonic extraction was concentrated to 15 ~ 20mL, 50mL ether standing after cooling add clarification supernatant. Fractional precipitation with methanol (20,10,5 mL) heated allows the dissolved, let cool to room temperature, filtered. Combined methanol solution, reduced to 15 ~ 20mL, let cool, add 50mL ether, shake, ibid treatment, combined methanol solution set is constant weight of evaporating dish, evaporated to dryness in a water bath,[link widoczny dla zalogowanych], dried at 105 ℃ to constant weight , the total sample yield of glucoside yield based on total glycosides /% a [Cordyceps extract dried powder mass (g) / dry mass Cordyceps (g)] x100. Table 1 Orthogonal test factor level L. (3) 3 Results and discussion 3.1 with water as solvent used when the results of orthogonal test L. (3) experiment, using water as extraction solvent, extraction times investigated (A), solid to liquid ratio (B), extraction time (C), ultrasonic power (D) of Cordyceps extract rate of the total glycosides. The results in Table 2. As can be seen from Table 2, with water as solvent extraction, ultrasonic extraction of total glucosides of Cordyceps order: extraction times> Ultrasonic Power> liquid ratio> extraction time, the best conditions: C. D. 3.2 methanol as solvent extraction using orthogonal test L. (3) experiment, using methanol as extraction solvent,[link widoczny dla zalogowanych], extraction times investigated (A), solid to liquid ratio (B), extraction time (C), ultrasonic power (D) of Cordyceps extract rate of the total glycosides. Results in Table 3.19 Phase 2 (Total 99) April 2009 CHINA specialty 'ForestBy-ProductandSpecialityinChinaNo. 2 (GSNO.99) Apr, 2009 flower explants Tissue Culture Study on Improvement of treatment Jian-Jun Han, SUN Hai (Heilongjiang Forestry Vocational Technology College, Mudanjiang 157011, China) Abstract: The flowering plants of various organs such as roots, stems, leaves and flowers,[link widoczny dla zalogowanych], and various organizations such as the cortex, and the formation of layer, stem pith, after in vitro under appropriate conditions, almost all in the callus, therefore, key to the success of callus induction is not primarily a source of explants and type,[link widoczny dla zalogowanych], but the culture conditions, of course, explants on the type and location of the plants on callus induction can not be ignored. Key words: tissue culture; explants; improved technology t the choice of material selection options 1.1 female plant phenotypic characteristics of the good traits, such as the female parent plant type, flower large and long flowering period, followed by vigorous growth in the period the year saplings; 1 month prior to the vaccination for disease control, vaccination should be placed before the 1 week 1 week under the sun and water control and other treatment, and then were cultured. 1.2 explants selected in accordance with the basic concepts of plant physiology, on the same plant in different organs have different physiological age,[link widoczny dla zalogowanych], the same organs in different parts also have different physiological age. Plants along the main axis, the more progressive parts of the growth of organs formed by the shorter the time, the physiological age of the older, more mature developmental approach, so this test the choice of material was collected, or the lower leaves of spring shoots new base of the sprout leaves. 1.3 Because the season of inoculation of plant has its own biological clock, and if people break it takes time and conditions, so we try to follow the laws of nature. In the end, or has entered a growth period of dormancy sampled explants in culture unresponsive or can not be successfully cultured; in the hot and rainy in the summer cultivation of selected materials, with the bacteria for more effective sterilization is difficult; only Sample the beginning of spring in the growth better. 2 Methods 2.1 The establishment of aseptic culture of plant tissue culture sterilization is the primary task, including the medium sterilization, sterile filtration is not heat-resistant material, heat sterilization and plant material with water as extraction Table 2 solvent calculations of total glucosides of Table 3 when using methanol as extracting solvent calculations of total saponins can be seen from Table 3, with methanol as solvent extraction, ultrasonic extraction of total glucosides of Cordyceps order: extraction times> Ultrasonic Power> ; extraction time> liquid ratio, the best conditions: ABCI) 3. 3.3 the best combination of confirmatory test results,, 2O, in order to test the reliability of the results of orthogonal test, using the optimal extraction conditions of the experiment . Water as extraction solvent, extraction rate of 5.629 of total saponins, with methanol as extraction solvent, extraction rate of 8.994 of total saponins.
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