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1.5 statistical methods to experimental data (± s) that, with the SPSS 11.0 statistical software for data processing, using single factor analysis of variance, pairwise comparison between groups with t test.
1.3 delivery methods Jujuboside A group was given Jujuboside A [Chengdu days provide limited liability company Futang economy (purity ≥ 98%)] 20mg/kg, nimodipine group received nimodipine injection 1mg/kg , sham operation group and model group received saline, both for intraperitoneal injection. 30min before the administration of group 1 patients, based upon the dose after 1 day 1. 12,24,48 four hours after ischemia, 4 days, 7 days,[link widoczny dla zalogowanych], from each of 6 rats brains were removed determination of the indicators.
Key words
Indicators and methods for detecting
Male animals
1.1 120 SD rats, weighing 200 ~ 230g, from the Guangxi Institute for Drug Control Laboratory Animal Center.
1.2 model with surgery group preparation and group Zea  Longa slightly modified method, the SD rats were prepared for the middle cerebral artery occlusion reperfusion model (MCAO), Operation Methods: SD rats, with 10% hydrated intraperitoneal chloral hydrate anesthesia (35mg/100g), fixed on the operating table supine, neck, skin preparation, routine disinfection of shop towels. Line neck incision, separated layer by layer and exposed to right common carotid artery (arteria carotis commun, CCA), careful separation of the right CCA, internal carotid artery (arteria carotis interna, ICA) and carotid artery (arteria carotis externa, ECA ), to avoid stimulation of the vagus nerve. In the CCA,[link widoczny dla zalogowanych], ICA, ECA, on the set a 3  0 silk thread, the ECA and the separation of the superior thyroid artery after the distal occipital artery (approximately from the common carotid artery bifurcation 5mm ~ 7mm), ligation of ECA. Subsequently, ICA and CCA were set at the microscopic arteries folder, away from the ICA, ECA bifurcation of the CCA on 3mm cut a \The blunt end of the nylon line into the CCA roast the small mouth, relax the arteries of ICA on the preset folder, the nylon cord from the CCA into the ICA, the forward Xu Jin 18 ~ 20mm, or stop into a little case of resistance. ICA will be preset in the threads on the truss, to prevent bleeding and emboli slide. Suture the skin, of surgery. Randomly divided into ischemia-reperfusion model group, nimodipine group Jujuboside A group, sham operation group were anesthetized by the above method, after skin incision and suture.
Abstract Objective: To observe the effect of jujuboside A (JuA) on amino acids levels and apoptosis in hippocampus cerebral ischemia in rats and to probe into the neuro-protection mechanism of mild hypothermia on cerebral ischemic injury. Methods: Using the model of focal middle cerebral artery occluded, the healthy Wistar rats were randomly divided into sham group, cerebral ischemia model group, nimodipine group,[link widoczny dla zalogowanych], and JuA treatment group. Every 6 rats in each group were sacrificed on 0.5d,[link widoczny dla zalogowanych], 1d, 2d, 4d, and 7d after cerebral ischemia and reperfusion to determine amino acids and apoptosis levels in the brain. Results: Compared with the model group, the nimodipine and JuA groups had glutamic acid and apoptosis significantly decreased (P 0.05). Conclusion: JuA can regulate effectively the release of amino acid and decrease apoptosis in the hippocampus after cerebral ischemic injury.
Jujuboside A on focal cerebral ischemia in hippocampus levels of amino acid and apoptosis


<div style=\ Abstract Objective: To observe Jujuboside A on cerebral ischemia and excitatory amino acids in hippocampus and apoptosis and to explore Jujuboside A on cerebral ischemia injury in rats. Methods: Rat middle cerebral artery occlusion reperfusion model of healthy SD rats were randomly divided into sham operation group, ischemia-reperfusion model group, nimodipine group Jujuboside A group, four in cerebral ischemia 12,24,48 hours after reperfusion, 4 days, 7 days, from each of 6 rats brains were removed, measured in hippocampus of rats with ischemic brain levels of amino acid and cell apoptosis. Results: In addition to the sham operation group, model group, nimodipine group and A group Jujuboside time points observed in hippocampus of ischemic brain glutamate (Glu) and apoptosis were significantly decreased (P 0.05). Conclusion: A can regulate Jujuboside hippocampus after cerebral ischemia in the release of the amino acid level, inhibition of apoptosis.
1.4.2 neuronal apoptosis in rats after decapitation, immediately remove the part of the right side of the brain tissue placed in neutral formaldehyde (pH7.2 ~ 7.4 phosphate buffer as solvent, formaldehyde PBS) and fixed for 12 hours at room temperature, from the hypothalamus in the 1 / 3 plane coronal slices, thickness 4mm, followed by dehydration, dipping wax, embedded, slice thickness of about 6μm, HE staining, light microscope to determine the location hippocampus near the paraffin at the continuous sections. Grilled sliced into 65 ℃ 6 hours on the machine, remove and put into 4 ℃ refrigerator for later use. In situ nick end labeling (TUNEL) method to detect apoptosis: the dewaxing tissue sections in order to carry out TUNEL staining, observed under light microscope of 400 times the average of 5 positive cells in the Perspective of the specific steps according to kit instructions.
Suanzaoren mainly used sedative. In recent years,[link widoczny dla zalogowanych], the main component Jujuboside found on ischemic brain injury A neuroprotective effect, but the specific mechanism is not fully understood. In this study, the rat model of focal cerebral ischemia, cerebral ischemia observed levels of amino acid changes in the hippocampus and neuronal changes and apoptosis in the two types of Jujuboside index of A to and from cells further explore the molecular level Jujuboside A possible mechanism.

1 Materials and methods
1.4
excitatory amino acids in cerebral ischemia and apoptosis Jujuboside A

1.4.1 amino acid end of the experiment brains were removed quickly, ice tray isolated cerebral cortex of the right frontal lobe, brain tissue preparation, said the wet weight, homogenized, supernatant after centrifugation HPLC fluorescence detection of glutamate (Glu), γ  aminobutyric acid (GABA) content.
Key words jujuboside A cerebral ischemia amino acids apoptosis
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