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Ginseng Fluorescence study of Chinese herbal medicines


Spectrophotometer with the software features can be asked of the operations spectrum. Difference spectra can be observed from the spectra of two different components or content. If the same source material, after deduction of the points in the 3D spectrum intensity is essentially zero; If the material from different sources, the spectra after the deduction,[link widoczny dla zalogowanych], will retain some of the display area of fluorescence, which play a role in identifying substances. Figure 9 is a spectrum of American ginseng and wild ginseng by subtracting 3D graphics, displays some of the fluorescent region also exist; Figure 1O is a spectrum of American ginseng and self-reference by subtracting 3D graphics, it also shows some of the fluorescent zone exists; Figure 1l the mountains in white ginseng spectrum subtracting 3D graphics, also show some of the fluorescent zone exists. 3D spectra of the three differences between participants with different compositions and material content reflects the different quality parameters, source of 34 of Instrument No. 5 of 2009 different, and thus play the role of differential class participation. Visible, three-dimensional fluorescence spectrometry is a very effective method. 3.4 Stability and reproducibility of spectral samples to study the stability and reproducibility of the spectrum,[link widoczny dla zalogowanych], respectively, parallel samples were measured parallel to the sample spectrum between the peak position, strength and stability, and showed good reproducibility and stability. 4 Conclusion The use of modern analytical techniques to obtain medicine information on the chemical composition of different individuals, expressed graphically, and the comparison and analysis, is the identification and characterization of Chinese herbal medicine the necessary technical means. This study showed that the three parameters of the water extract contains a variety of the same or different fluorescent substances, their chemical composition differences in the type and content, because of growth conditions, different environments, and production preparation process there are differences, the various Characteristics of participants or the intensity of fluorescence emission spectra showed differences between the different classes as argument and its derivative spectrum fluorescence spectroscopy, synchronous fluorescence spectroscopy, fluorescence spectroscopy has a characteristic three-dimensional, fingerprints, thereby to achieve the rapid identification of parameters, identification and quality control. Wears the conventional two-dimensional fluorescence spectra comparison, derivative fluorescence spectroscopy can enhance the clarity and determination of the secondary spectral peak is very beneficial to the weak, to better reflect the subtle changes between the spectra, separation of the overlapping fluorescence peaks, thus increasing spectral resolution, spectral differences protruding, enhanced spectral recognition. Simplified map of synchronous fluorescence spectroscopy, the analysis simple. Three-dimensional fluorescence spectrometry,[link widoczny dla zalogowanych], can quickly give a more complete spectral information is a valuable spectral fingerprint technology. Identification of three methods of using the above reference class, extends the parameters of class methods and means of identification, identification of the reference class is more rapid, simple, more scientific and practical value. References 1 Chen Junhui, Ming-Yong Xie and so on. MATERIA MEDICA. 2005,3 (9) :845-8472 Sun Lihua, Zhuang anthology. Chinese pharmacists. 2000,[link widoczny dla zalogowanych],4 (3) :248-2493 Xu Hui,[link widoczny dla zalogowanych], Zhu Billiton. Spectroscopy and Spectral Analysis. 2007,27 (7) :1381 -13,844 Xu Guang Tong, Yuan Hongfu, Lu Wanzhen. Spectroscopy and Spectral Analysis, 2000,2 O (2) :134-1425 Xu Jingou, Zun this. Fluorescence analysis (third edition). . Beijing: Science Press ,2006:137-140 ,154-1556 Xu group, Li Xingwang and so on. Analysis. 2007,26 (4): 484 ~ 487 Received date :2009-04-13Studyonidentifyingmedicinalginsengyfluorometricmethods. FangHuimin (ExperimentandTech-nologyCenter, AnhuiUniversity, Hefei, 230031) Medicinalginsengswereanalyzedandidentifiedbyderivativefluorometry, synchronousfluorometry, andthreedimensionalfluorometry. Usingthesemethods, thedifferencebetweenspectraofginsengscanbeclearlyidentified, andthecharacteristicsoffingerprintpatternsareobviouslyexhibited. Theresultsprovideascientificreferenceforrapidlyidentifyingginsengs.
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