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Wysłany: Pon 4:44, 04 Kwi 2011 Temat postu: ghd stijltang 番茄组࣌ |
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番茄组培苗的不同阶段对抗生素和PPT的抗性筛选试验
entmass—concentrationsduringthestagesofthecallusformationoftomato’Scotyledonandhypoeotyl,thedifferentiationandgrowthoftomato’Sbud,andtheroot—growthoftomatopla—nfle~,SOthattheoptimumscreeningmass—concentrationsneededbygenetictransformationoftomatocouldbedetermined.Theexperimentshowedthattherewasnotdistinctdifferenceinresistancetoabovereagentsbetweenthetwoself-bredlints—T79andT151;ateachstageoftomatotissueculture。bethkanamyeinandPPrrhadaverystrongerinhibitiononexplants.whileneomycinhadnoobviousinhibitiononthem,ghd stijltang;ontheotherhand,moncler outlet,duringthedifferentstages,thereexistedobviousdifferenceindifferentexplants’reactiontokanamyeinandPPrr,andparticularlythecallusformationofhypoeotylWasthemostsusceptibletoKan。thoughthecallusformationofcotyledonandtheroot—growthofplantletswereunsusceptibletoit;inaddition,thecallusformationofhypoco~andthedifferentiationofbudsweresusceptibletoPIyr,ecco shoes,thoughthecallusformationofcotyledonWasunsusceptibletoit.Hence,itWassuggestedthatforgenetictransfor-mationoftomato,Kan100nag,ghd stijltang kopen。L~shouldbeselectedasidealscreeningmass—concentrationatthestageofhypoeotylcallusinducingandplantlets’root—growth.Kan50nag。L~atthestageofhypoeotylcallusin—ducing;andKan70mg。L~atthestageofbuddifferentiation.However。forPPT,1.0mg·L一。shouldbetakenasidealscreeningmass—concentrationatthestageofcotyledoncallusinducing。0.5mg。L一。atthestageofhypoeotylcallusinducing;and0.75mg。Latthestageofbuddifferentiationandplanflets’root—growth.Keywords:tomato;tissueculture;pre.testinresistance,supra for sale;antibiotics;herbicidePPrr
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